Yes, you must collect the samples using all of the swabs provided in the kit.
If you order an ancestry, health or trait test, each kit will contain 4 swabs. You must collect all 4 swabs from from one single individual.
If you order a relationship test, such as a paternity test for one alleged father and one child, you will receive two separate kits, one kit for the alleged father and one kit for the child. Each relationship testing kit contains two swabs and both swabs must be used for sample collection.